Normalized Elution Time (NET) Prediction Utility Compute predicted normalized elution time values for peptide sequences
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Normalized Elution Time (NET) Prediction Utility Ranking & Summary

Normalized Elution Time (NET) Prediction Utility Tags

predict calculate Normalized VBR MP3 peptide viewer peptide compute time difference peptide fragmentation peptide sequence peptide information Peptide fragment analyzer predict peptide fragmentation analyze peptide peptide distribution peptide hit result peptide modeller peptide extractor peptide identification MALDI peptide peptide statistics proteotypic peptide peptide evaluation peptide aligner align peptide normalized elution time NET prediction GeneSpring peptide fragments add time values peptide analysis

Normalized Elution Time (NET) Prediction Utility Description

Normalized Elution Time (NET) Prediction Utility is a small utility designed to calculate the predicted normalized elution time (NET) values for a list of peptide sequences. The elution times are intended for use with reversed phase liquid chromatography (e.g. using C18 coated silica particles) analyses, and are used in our lab for alignment of LC-MS and LC-MS/MS datasets to one another (using the predicted elution times to form a master in-silico dataset to align each dataset to). The predicted elution times are also used by the Protein Digestion Simulator Basic when calculating the number of uniquely identifiable peptides within an input file using both mass and NET. The NET Prediction Utility reads the peptides from a text file containing one peptide sequence on each line. The output file includes the peptide sequence plus the predicted NET value (and/or predicted SCX value) using one or all of the prediction algorithms: · Kangas/Petritis LC retention time model · Kangas/Petritis SCX fraction model · Krokhin hydrophobicity model · Mant hydrophobicity model

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